Abstract: The combination of internet and traditional educational means has become a new teaching model in the field of modern education. Taking the course of microbiology experiment as an example and under the background of “Internet+”, this paper explores the preview model revolving around the “micro-class and self-check”, integrates the intelligent teaching APP into experimental teaching, and uses virtual simulation system as a complement to the extracurricular experiments. The laboratory safety access system and the laboratory assessment and scoring system are combined to realize the reform and practice of experimental teaching model by covering from “pre-class”, “on-class” to “after-class”. On the one hand, the reform of experimental teaching model based on “Internet+” technology can increase students’ enthusiasm for preview and efficiency of experimental skills mastering, and improve their understanding of the two-way transformation and promotion between the theory and practice in biology. On the other hand, it will be useful for teachers to better manage and control the course, and establish a more comprehensive and science-based evaluation system of experimental course.