Abstract: In order to make up the lack of immediacy and large-scale equipment in the PLC experiment, the virtual simulation experiment of material production, classification and assembly is designed based on Factory IO. The model built by Factory IO can communicate with PLCSIM and real PLC, control the model action in TIA Portal15.1 programming environment, and realize the joint debugging of TIA Portal and Factory IO. The test shows that the experiment can realize the production, classification and assembly of blue bottom, blue cover, green bottom and green cover, supplement PLC practice teaching, improve the experiment design phase and stimulate students' innovation abilities.