Abstract: The innovative experiment project of mechanical principle plays an important role in improving the ability of mechanical innovation. The construction of innovative experiment project lays the foundation for setting up innovative experiment. In this paper, the thought of “one body, two wings and two guarantees” for innovative experimental project is established. The experiment guide is the body, besides the conventional content optimization, the experimental guidance also needs to guide the application of innovative methods in the experiment. Teaching methods and innovative methods are the two wings. The process of research-based learning and research-based teaching helps to enhance students’ experimental autonomy. Assessment method and experimental teaching management are two guarantee mechanisms. Formative assessment method and continuous improvement mechanism of experimental management based on conjugate analysis are constructed to ensure the realization of experimental teaching objectives.