Abstract: Self-designed experiments can improve students’ innovative spirit and practical ability. In the experimental teaching of animal physiology, this paper carries out experimental teaching practice based on self-designed experiments, explores the implementation strategy of self-designed experimental teaching for undergraduates, analyzes the influence of self-designed experiments on students’ learning attitude and learning effects; establishes evaluation methods of self-designed experiments, and improves the evaluation system of experimental course. The practice shows that self-designed experimental teaching has significant effects on stimulating students’ initiative of experimental operation and innovative thinking, and its implementation can form a student-centered experimental teaching system, and achieve the ultimate goal of cultivating students’ innovative and practical ability.