Abstract: In view of the realistic demand of cultivating innovative engineering talents with multi-disciplinary integration under the emerging engineering education. Combined with the the present situation of civil engineering experiment teaching in Southwest University, this paper proposed an innovative experimental project of antimicrobial hybrid fiber reinforced concrete which has the characteristics of research, design, comprehensiveness and cross-discipline. To mix the fiber and antimicrobial agent into ordinary concrete material, and analyze the influences of them on the mechanical and antimicrobial properties of concrete, then obtain the optimal mix ratio. So as to ensure the safety and sanitation of fiber concrete. This experimental project has a reasonable experimental content design, and throughout the idea of multidisciplinary integration. This way can fully expand students’ subject vision, stimulate students’ interest in engineering practice, cultivate students’ innovation ability, and establish the engineering consciousness with safety and health as the priority. The achievement greatly provides reference for the reform and innovation of civil engineering material experiment teaching.