Abstract: Plant growth in different studies about research greenhouses have different requirements for light intensity and illumination time, however, traditional lighting equipment and methods are hard to achieve differentiated light supplement and difficult to meet unique requirements for plant light environment in research greenhouse. This paper, in accordance with the practical application requirements of light supplement in research greenhouse, has selected the LED light source suitable for plant growth, and simulated with Dialux software. Meanwhile, an automatic light supplement system which features uniform light distribution, controllable light intensity and illumination time, safe operation and energy saving is designed. The system has fully considered the requirements of light environment of main cultivated plants in research greenhouse, and the requirements of management and users for automatic control of light supplement system in actual operation.