Abstract: This paper discusses two strains of yeast, 2S-6 and HN-3B, which are screened from collected soil samples from different habitats. 2S-6 and HN-3B can produce oil by using pectin biomass or its products, corresponding to the oil content of 51.44% and 64.66%, respectively. The 2S-6 strain has been identified as Kodamaea ohmeri through molecular identification, and it also has excellent ability to decompose pectin, indicating that it has strong pectinase activity and can be used for the construction of enzyme membrane reactor for pectin degradation. Therefore, a large amount of non-glycosylated polygalacturonase was obtained through genetic engineering technology. Under the optimal conditions of 2.55 hours cross-linking treatment with 1.25% glutaraldehyde, it was fixed on the chitosan nanofiber membrane of electrospinning to prepare the enzyme membrane reactor, and pectin was hydrolyzed into galacturonic acid monomer with a conversion rate of 63.96%. This will lay a foundation for realizing the resource utilization of pectin biomass waste and reducing environmental pollution.