Abstract: As a relatively independent chromatography method, ion chromatography has been introduced into undergraduate experimental teaching. In this experimental teaching design, ion chromatography was applied to analyze common anions in three different water bodies, including rainwater collected in pipeline, surface runoff and pond water in the campus. In order to stimulate students' interest in learning, several teaching methods were applied. The experimental principle was explained according to the structure of the instrument, which combined theory with practice. The students were encouraged to participate in water sample collection and were divided into several small groups to operate the instrument during the experiment. The experimental data were shared for adequate analysis. During the operation of the instrument, the students were required to answer several questions by carefully observing the structure of the ion chromatograph and the process of sample analysis, which greatly motivated their initiative. This experimental achieved good teaching results.