Abstract: The feedback of teachers and students on the actual use of laboratories in universities and colleges is very important. In this paper, with reference to the test methods required by Standard for Indoor Environmental Pollution Control of Civil Building Engineering, different types of laboratories in a university in Shanghai were measured, and a more comprehensive statistical survey was conducted through questionnaires. The basic information, usage and management situation, the health of the experimenters, and the feelings and evaluations of teachers and students were comprehensively counted. The results show that various types of laboratories have different degrees of air pollution, and hardware facilities and pollution protection need to be improved. In response to the above problems, combined with the opinions of teachers and students, it is recommended that laboratories in universities and colleges should have reasonable ventilation systems, complete protective measures and scientific management models to effectively protect the personal safety and health of teachers and students.