Abstract: Experimental organic chemistry, which is the core part of organic chemistry course, is a basic course for students from pharmaceutical major. In order to encourage students to observe, analyze, and solve the practical problem, some exploration, reform and practice of experiment in experimental organic chemistry are carried out in the aspects of teaching content, teaching mode and student examination score evaluation. These teaching reforms have greatly mobilized students’ learning initiative, stimulated students’ learning interest and cultivated students’ innovative ability. The feedback shows that good teaching results of these approaches were achieved.