Abstract: For the professional experimental technicians and scientific researchers, it is the key point to obtain an ideal test result that applying infrared instrument analysis technology reasonably. In this paper, by means of literature review and experimental study, some common and typical problems in the infrared spectrum testing are discussed in combination with practical cases, and the content includes the principle of attenuated total reflection (ATR) test, the high wave number sensitivity attenuation, the feasibility of non-KBr potassium tablets, the standardization operation of KBr potassium tablets, the quality evaluation and optimization of infrared spectra, etc. In view of the above problems, some solutions and suggestions are put forward in this paper to promote the application of infrared analytical technology, which are of great significance to the improvement of the application of infrared spectrometry technology and the experimental teaching.