Abstract: In view of the characteristics of agroforestry botany experimental teaching, such as various biological species, long experimental time span, and biological security risks, this paper analyzed the necessity of building a virtual simulation experimental teaching center of agroforestry botany, and explained the strong support of the school’s high-quality agroforestry achievements and experimental teaching resources for the construction of the virtual simulation experimental teaching center. It also introduced the transformation of high-level scientific research results into undergraduate experimental teaching content, the establishment of provincial-level virtual simulation experimental teaching center and the construction practice of national virtual simulation experiment teaching “golden course”. Practice has proved that the virtual simulation experimental teaching center has achieved remarkable results in the aspects of personalized autonomous learning, high-quality experimental teaching resources sharing and so on.