Abstract: A virtual simulation experiment of single-cylinder internal combustion engine is developed for the requirements of talent cultivation in intelligent manufacturing. The virtual simulation technique has been more and more applied in the comprehensive experiments, especially under the background of new engineering and technical disciplines. The contents of virtual simulation experiment include mechanical structure, operating principle, characteristics of key components, technological processes of disassembly and assembly, mechanical analysis, etc. The experiment is conducted by teaching video, text instruction, engine model, and physical display. Firstly, the students can conduct the model cognition and the theoretical study based on basic learning module of the virtual simulation platform. Then, the students turn into the module of mechanical structure and analysis, which includes the training of software operation, the practice of disassembly and assembly, the test of theoretical knowledge, the force analysis of mechanical parts. Finally, the experiment report module can record the experiment process, give the marks, and export the full report. The teaching effect and the experiment efficiency have been well improved by the combination of virtuality and reality together with the way of online to offline. Besides, the instructional design also provides significant support for practical teaching and ability training.