Abstract: In order to overcome many problems in the practical teaching of large-scale instrument analysis and testing in colleges and universities, such as insufficient teaching resources, high teaching cost, limited teaching method and low occupancy rate of instrument in the scanning electron microscope analysis testing, a simulation interactive platform for scanning electron microscopic analysis experiment teaching was developed by using virtual simulation technology. With no need to enter the laboratory, students can autonomously learn the relevant theoretical knowledge of scanning electron microscopic analysis and testing, complete the simulated interactive operations of sample preparation, instrument testing and analysis by using the fragmented time after class, which lays a good foundation for the hands-on operation. Students can verify their knowledge through online simulation tests and provide timely feedback to their teachers to promote continuous improvement of the curriculum. The teaching practice shows that the combination of scanning electron microscopy virtual simulation experimental teaching system and physical experimental teaching breaks through the limitations of traditional experimental teaching, arouses students’ interest in learning and initiative in independent learning, makes the teaching process more safe and efficient, and improves the teaching quality. This virtual simulation experiment teaching system helps improve students’ subjective initiative and practical innovation ability, and provides a new practical teaching mode for the cultivation of innovative talents in functional materials research and development industry.