Abstract: Based on the training requirements of innovative and practical ability of students, a research-oriented and comprehensive experiment has been designed and explored in teaching of bioreactor technology course. This experiment focuses on effects of culture model on growth and metabolism of animal cells. This experiment includes experimental units of culture model, kinetics of cell growth and metabolism, and parameter measurements, covers the knowledge and ability training of kinetics of biological reaction, instrumental analysis, data analysis and processing as well. And it cultivates students’ ability to expand and innovate in using experimental data for bioreactor design. Through the teaching design and exploration by teachers, students’ enthusiasm for research and innovation were inspired, and students’ innovative ability and engineering ideas were cultivated in a simulated research-oriented environment in this experiment.