Abstract: Large-scale instruments and equipment are important conditions to guarantee experimental teaching, scientific research, personnel training and social services in colleges and universities. Open sharing is an important way to improve the using efficiency of large-scale instruments and equipment, and service teaching, scientific research and society. Based on the analysis of the general situation of the construction and application of large-scale instruments and equipment, the innovative measures for the open sharing work under the background of the first-class disciplines of the country are proposed on the basis of explaining the necessity of the open sharing of large-scale instruments. The measures are explained in detail from five aspects: improving the management system of large-scale instruments and equipment, construction of open and shared platform, large-scale instruments and equipment open and shared platform training construction, construction of experimental teaching staff, and establishing a new incentive mechanism for the use of large-scale instruments and equipment, and the resources of large instruments will be full used.