Abstract: The space optical payload experiment needs to be carried out in the environment with clean, dark and apposite temperature, humidity and pressure conditions. In order to make the simulated experimental conditions closer to the space environment, especially the light environment, a set of multi-parameter sensing system, which is suitable for space optical payload imaging and stray light testing, is established for the real-time monitoring of data (dust particle count, ambient light distribution, temperature, humidity and air pressure) in the class thousand clean laboratory. Based on the LabVIEW controlling program, the sensors are managed through serial communication, and the environmental condition data are collected, stored and comprehensively analyzed. Tests show that the monitoring system is capable of real-time monitoring and comprehensive analysis for dust particles and atmospheric condition under weak light conditions, and the monitoring capability of test automation of optical load and the reliability of calibration data is improved.