Abstract: A virtual simulation experiment platform for 6-DOF robot is developed by combining the realistic 3D image display function of the robot simulation software V-REP (virtual robot experimentation platform) and the powerful computing ability of MATLAB program. Firstly, the robot model is built in V-REP. Then the communication mechanism and communication call mode of V-REP are introduced. Finally, the simulation of robot joint space trajectory planning and Cartesian space line planning are used to illustrate how to use the MATLAB program to realize the motion simulation of the robot inside V-REP. The robot simulation technology is used to build an interesting experimental platform, which is applied in the experimental classroom of robot basic principle. The experimental teaching mode of flipped classroom is explored to improve students’ learning interest and robot design simulation ability.