Abstract: Large scientific research instruments are a manifestation of the advanced science and technology, an important prerequisite and hardware guarantee for cultivating innovative talents, and a precious resource for conducting high-level experimental teaching. In order to promote the cultivation of innovative research talents and expand the content of undergraduate experimental teaching in biology and medicine, this study has designed a specific teaching plan for undergraduate experimental teaching around a flow cytometer. In order to address the classic “cell proliferation/cell cycle” problem, two different principle flow cytometric techniques are designed as comparative experiments. The practice has shown that teaching comparative experiments using a flow cytometer can deepen undergraduate students' understanding of the large scientific research instruments, cultivate their solid hands-on abilities, and stimulate their innovative thinking and research enthusiasm.