Abstract: Under the background of “new engineering” with discipline integration, a practical training course on electroencephalogram (EEG) processing and analysis for the senior undergraduates majoring in biomedical engineering is set up. In this experiment, the multi-channel EEGs of healthy subjects under orienting in attention network are taken as the research objective, and the spatial distribution of EEG characteristic band power is analyzed, to provide support for the study of the mechanism of attention. Through this experiment, students are required to apply theoretical knowledge to practice to master the skills in data preprocessing, time-frequency analysis, frequency band extraction and power distribution of EEG signals, which improve their ability to analyze and solve problems independently. Furthermore, the application of scientific research to undergraduate experimental teaching can broaden students’ scientific horizons, improve their interest in scientific research, and exercise their scientific research thinking. It is definitely helpful to cultivate high-quality compound “new engineering” talents needed by emerging industries in the future.