Abstract: Large-scale instrument is featured as expensive and sophisticated, and a highly careful operation and high maintenance cost are required. Furthermore, high openness of the large-scale instruments and strong mobility of persons delivering sample make the safety management for large-scale instrument laboratory much more complicated. In order to ensure the security of laboratory with above mentioned characteristics, a series of steps has been taken by School of Materials Science and Engineering, Nankai University in recent years. They did a great progress on standardizing the traditional management modes, moreover, a lot of new appearing technology and scientific programs have been innovatively, gradually explored and performed. After a couple of years’ practice and implementation, a general large-scale instrument room safety management scheme has been established, that is, “collaboration of different regions, combination of virtual and real, remote assistance, joint prevention and control”. Based on the above management practices, the platform has been operating efficiently and openly for several years, providing strong support for the cultivation of high-quality talents, high-level scientific research, and the construction of “Double first-class” disciplines.