Abstract: In view of the problems faced by the safety management of basic chemistry laboratories in colleges and universities, such as insufficient informatization, insufficient safety awareness of laboratory personnel, and limited investment in laboratory safety, this paper takes human management as the core and builds an efficient, convenient, flexible and low-cost security access system for basic chemistry laboratories in colleges and universities based on the Internet of Things (IoT) technology. The laboratory safety access system consists of three subsystems, namely, laboratory safety access test, laboratory access control and laboratory practice drill. In the operation of the laboratory’s security access system, the customs clearance mode is used to promote flexible and active learning of security knowledge, the IoT technology is used to support automatic real-time check-in for access control, and the combination of online and offline methods is used to complete the efficient and safe training, fully ensuring the fire safety and environmental safety of the basic chemistry laboratory. Through two years of practical application, the implementation of the system has realized the transformation of basic chemistry laboratory safety education in colleges and universities from passive assessment to active learning, and has achieved an improvement of laboratory safety management and education from the quantitative change to the qualitative change.