Abstract: Guided by experimental teaching combined with the requirements of easy availability of experimental materials, clear experimental process, stable experimental results, and short experimental time, the experimental teaching design for identification of protein ligand interaction by non-variable property spectrum technology is constructed by means of experimental process optimization, core consumables homemade, dynamic evaluation system construction, etc., to solve the difficulties in the application of large instruments and equipment in experimental teaching. Through the introduction of the latest experimental technology in the professional hot field, the experimental project broadens the horizon and enhances the sense of identity, which is conducive to the improvement of students’ majoy core quality.