Abstract: The interactive video virtual program is a computer application that utilizes video as the central medium and seamlessly integrates various media forms such as images and text. Depending on the user's operations, the program can provide different feedbacks. It boasts several key advantages, such as a streamlined production process, minimal equipment requirements, and an engaging content presentation that captivates learners. It can play an active role in experimental teaching. In this study, we developed a program using the Unity platform for the molecular identification experiment of traditional Chinese medicine, and we implement it in the molecular pharmacognosy experiment course at our college. The findings demonstrate that this interactive video virtual program effectively enhances teaching quality and yields satisfactory educational outcomes.