Abstract: Membrane separation is a green separation technology. Based on “green chemistry”, the ultrafiltration membrane separation experiment is improved from several aspects such as the selection of material and chromogenic agents, the experimental scale, the dosage of reagents, and the recovery of the waste liquid. A series of effective measures have significantly improved the experiment economy and greening. The implementation of the improved “the ultrafiltration membrane separation experiment” can not only improve students' professional quality, establish environmental protection and green chemistry awareness, but can also serves as a typical case of ideological and political teaching in green chemistry