Abstract: Against the backdrop of the “discovery and synthesis of quantum dots” recognized by the 2023 Nobel Prize in Chemistry, a comprehensive experiment on the preparation and application of perovskite quantum dots was designed to enhance the comprehensive experimental capabilities of undergraduates in chemistry and materials-related disciplines and to broaden their research horizons. This experiment covers key concepts including inorganic nanosynthesis, physicochemical mechanisms, characteristics of organic ligands and solvents, and instrumental analysis methods. It visually presents profound basic principles, allowing students to experience the joy of science through practice. The experiment aims to strengthen students’ understanding and application of professional knowledge, cultivate an objective, rigorous, and truth-seeking scientific attitude, and deepen their recognition of the important role of science and technology in national production and public health. Furthermore, it seeks to improve students’ awareness of environmental protection and social responsibility, further stimulate their enthusiasm for scientific research, and guide them to dedicate themselves to research practice in the future.