Abstract: Surface microbial inactivation is a long-term social demand, and low-temperature plasma inactivation of surface microorganisms is a hot research topic in surface microbial inactivation. Aiming at the hot spot, the latest research results of a new type of low-temperature plasma disinfection and sterilization were introduced into the comprehensive experiment of environmental engineering. Inactivate bacteria、SARS-CoV-2 (2019-nCoV) Spike pseudo virus were selected as inactivated objects in the experiment, and the research on the inactivation of microorganisms on the surface of objects by atmospheric pressure low-temperature plasma was carried out. The main research conclusions show the disinfection and sterilization efficiency of bacteria reached 99%, while the action time was less than 14 s. While the action time for SARS-CoV-2 (2019-nCoV) Spike pseudo virus was longer, which is 1 min at 23 ℃ and 4 ℃, and 3 min at −20 ℃. The results show that atmospheric pressure low temperature plasma technology is suitable for the inactivation of surface microorganisms such as food packaging in the cold chain industry. The experiment improved the students' ability to carry out experiments on low-temperature plasma and environmental engineering microbiology, but also reflected the high level of experimental content, and stimulated students’ enthusiasm and interest in experimental science.