Abstract: The experimental course teaching is an important part of college teaching, and an effective way to cultivate students’ professional quality and improve students’ practice ability. Under the Outcome-Based Education（OBE）concept, the teachers of Biochemistry and Molecular Biology Teaching and Research Section of the College of Life Sciences at Inner Mongolia University have carried out a teaching practice for Large-scale Biotechnology Experiment based on the already constructed and established Comprehensive Large-Scale Experiment Course for Undergraduate Biotechnology, with the aim of cultivating high-quality innovative talents. A mature experimental teaching model has been established to effectively stimulate students’ enthusiasm for experiments and initiative in experimental learning, and enhance their ability in independent exploration and practical problem-solving. Meanwhile, this teaching model can also further improve students’ comprehensive literacy and ability, thereby effectively improving teaching quality. These outcomes positively contribute to the cultivation of high-quality innovative talents.