Abstract: The water content in leather is a crucial indicator for evaluating leather performance. Thus, accurately determining the water content in leather holds great significance for ensuring leather quality. In this study, a Karl-Fischer-oven sample changer method was established to determine the water content in leather. The water content in leather was achieved rapid and accurate determination by optimizing experimental parameters such as sample mass, drying furnace temperature and carrier gas flow rate. Under conditions of 0.10 g sample mass, 140 ℃ drying furnace temperature and 50 mL/min carrier gas flow rate, the relative standard deviation and recovery of this method were 1.59% and 94.20% ~ 101.78%, respectively. Besides, the results closely aligned with those obtained by the oven drying method, providing reliable and objective water content in leather samples. When the water content in leather samples was 4.50%, the Karl-Fischer-oven sample changer method took less than 2.3% of the time required by the oven drying method, demonstrating its significantly reduces the testing time and greatly improves testing efficiency. These make it suitable for batch testing of samples in professional courses like "Leather Analysis and Testing" and "Leather Production Practice". In addition, this method can not only measure water content in leather samples from low water content (4.50%) to high water content (70.14%), but also determine the water content of different chrome-free tanned leather samples. This versatility makes it valuable for supporting basic data in clean tanning and new leather-based materials, which can be used in professional courses “Leather Chemical Materials”. This experiment improved students' participation in the course, and students can actively engage in solving practical tanning problems, thus fostering their hands-on practical ability, inspiring their enthusiasm in scientific research, laying a solid foundation for them to use scientific approaches to solve practical problems.