Abstract: The open sharing of large-scale instrument represents an effective approach for universities and research institutes to strengthen the cultivation of innovative talents, enhance research capabilities, and serve national strategic demands. To achieve efficient utilization and scientific management of large-scale instruments while maximizing asset benefits, this study draws on the management practices of large-scale instruments and equipment at the Key Laboratory of Mariculture, Ministry of Education, Ocean University of China. It explores the construction of a sustainable open-shareing platform for large-scale instruments, leverages artificial intelligence to improve virtual simulation training systems, establishes a professional and experimental technical team and improves the management and operational mechanism of the platform. It provides new concepts and methods for promoting the open-sharing of large-scale instruments in university laboratories.