Abstract: In order to enable bioscience students to gain a deeper understanding of the scientific research practices in plant disease resistance physiology and expand their knowledge of related techniques and research methods, the course teaching team has developed a comprehensive and designed experimental teaching project on plant disease resistance physiology, utilizing large-scale precision instruments such as modulated chlorophyll fluorescence imaging systems. Through the integration of scientific research into teaching, this project reasonably incorporated scientific research techniques such as pathogen activation and inoculation, chlorophyll fluorescence parameter measurement, and reactive oxygen species staining detection into the experimental teaching process. This enriched the content of undergraduate bioscience comprehensive experimental teaching, achieved a deep integration of theoretical knowledge and practical application, and effectively improved students' scientific research innovation ability and comprehensive scientific literacy.This project explores a new teaching path guided by cutting-edge scientific research content and driven by advanced instrument platforms, providing practical cases for the cultivation of top innovative talents in biology in the new era.