Abstract: Pernicious element sort of peril in university chemistry laboratory is much and the distribution place is vast and it is great to endanger. We analyzed the dangerous element of university chemistry laboratory, and referred Heinnich industry mishap precaution rule. Combined with unite personally practice, we sum up out the chemistry laboratory precaution mishap and safeguard security 3E’s countermove, which have the reference sense to secure quality advancing the chemistry laboratory in university.