Abstract: In view of the present situation of plant physiology experimental teaching and existing problems, contracted the requirements of experimental teaching and the training objectives, combined with the actual situation of teaching in Agricultural Colleges, we put forward the plant physiology experimental teaching reform measures, for examples, compiling the workbook, using multimedia teaching condition, improving laboratory management and changing the experimental courses evaluation method and other teaching methods. Through cultivating comprehensive quality of students, we optimize the experimental content, mobilize the enthusiasm of students and at the same time, improve the efficiency and level of plant physiology experimental teaching.