Abstract: We performed reformation for experiment teaching mode to reform the teaching mode of public health laboratory course. Questionnaires were taken to assess the effect of this study. This reformation can stimulate interest in learning, inspire positive thinking, consolidate the relevant theoretical knowledge, organization and coordination ability, improve the spirit of cooperation and field survey data collection ability, cultivate ability to analyze and solve practical problems, et al. Reformation of experiment teaching mode can cultivate students’ scientific quality at the same time, also cultivate the students’ practical ability and comprehensive quality, which are worthy of generalizing to other public health classes.