Abstract: The basic functions and the main features of simulation software Proteus were introduced in this paper firstly, and then some useful exploration and practice based on this software, including Single Chip Microcomputer (SCM) experiment teaching, curriculum design and subject competition, were described in detail. Furthermore, some specific applications in SCM experiment teaching were illustrated as much detail as possible. It is proved by practice that the applications, which are SCM experiment teaching with simulation software Proteus, are conducive to stimulate the students' motivation and initiative, and benefit to improve students' creative ability and practicable ability.