Abstract: Cytobiology is a subject with strong practicality. With the development of information technology and modern educational technology, digital microscope reciprocal system has gradually been applied to the experimental teaching of cytobiology, and embodied its advantages. With several years practice, we formed a set of methods of cytobiology experimental teaching by using the digital microscope reciprocal system, and achieved remarkable effects. Deeply application of this system will also vigorously promote the reform of cytobiology experiment teaching, and will provide a solid platform for developing the applied and creative ability of students.