Abstract: Biological safety cabinet is used in the handling of hazardous microorganisms box-safety negative pressure air-purifying equipment, which is to avoid exposure to infectious aerosols that may arise during the operation and splashing out on people, samples and environmental hazards and biological safety protection device design. But it does have the biological safety cabinet does not mean you have a “safe”, so we have to control and handle its application, with the ability to wield it, in order to effectively perform their biological security, reduce biological hazards factors escapes maximum guarantee the safety of operators, experiments and experimental environment.