Abstract: The use of artificial climate chamber in vivo and leaf disc method in vitro were inoculated for six species of powdery mildew pathogen identification of physiological host conducted powdery mildew inoculation test. The results of two methods are consistent with the identification of host resistance, and it can accurately distinguish six powdery mildew pathogen differentiating host powdery mildew of physiological races 2F sense resistance. In addition, using in vitro leaf disc inoculation, the difference of disease index between the susceptible hosts was not significant, while the use of artificial climate chamber in vivo inoculation, a significant difference of disease index among susceptible hosts were observed.