Abstract: With the maturity of the cloud computing technology, desktop cloud makes applications more secure and flexible. In this paper, we analyze the defects of traditional computer laboratory management, and combined with computer laboratory teaching environment construction and application practice. This method integrates computing power and data storage by cloud computing and virtualization. We also build the experiment teaching environment based on FusionAccess by the technology of desktop cloud, and analyze the feasibility and reliability of the desktop cloud technology. The construction and management of computer laboratory have also been improved significantly.