Abstract: The normal universities are in the important transformation period of structural adjustment. In the new reform condition of our university, there were many problems in the traditional curriculum of biochemistry experiment. According this situation we tried to restructure the teaching system, optimize the teaching content, reform the teaching methods and approaches, and establish the scientific and reasonable evaluating system. In the whole teaching process, we attached great importance to the students’ actual demands and worked hard to encourage their interest for learning and raise their comprehensive quality.