Abstract: The culture construction is an important part of the experiment center construction, which includes very large areas. Combined with the process of cultural construction in mining engineering experiment center, Henan Polytechnic University, the concept of experiment construction center was introduced, the construction content from the system cultural, environmental cultural human culture construction also were introduced. With the culture of experiment center, a patron of learning and an education-oriented environment were created, the students’ interest in the experiment was stimulated, and the students’ practice ability and the sense of innovation were greatly enhanced. It can provide a reference to create culture construction for other university laboratory.