Abstract: Paclitaxel is a highly effective anticancer drug which is mainly extracted from Taxus species,it can be produced by cell culture technology. This study works on the effects of culture conditions such as the types of explants,the types and concentrations of hormones,illumination conditions on callus induction of Taxus media,in order to find out the most suitable culture conditions for Taxus media callus. The results showed that in the experimental conditions,the most suitable hormone combination for Taxus media callus induction was 0.5 mg / L NAA+0.2 mg / L 6-BA+2 mg / L 2,4-D.The most suitable explant was the stem with buds; Dark culture was more conducive to callus growth compared to light culture. This project can provide some references for the production of paclitaxel by cell culture.