Abstract: In order to carry out the teaching reform guidelines of Sichuan University on the construction of a number of comprehensive,design-typed experimental courses and innovative exploration (entrepreneurial) experimental courses ,scientific research were combined with experimental teaching in this paper.According to some research achievements,an innovative instrumental analysis teaching experiment——Carbon Nanodots(CDs)-based fluorescent spectrometry for detection of glutathione(GSH),was explored and designed.This experiment is stable,reproducible and innovative.It can be used as a teaching experiment of instrumental analysis experiment.Meanwhile,there are many opening experiment contents and research topics including different preparation methods of carbon nanodots,the correlativity between the size and fluorescence characteristic of carbon nanodots,the design of fluorescent probe,and other target analysis,therefore,the experiment can be used in opening comprehensive chemical experiment teaching project.