Abstract: In order to study the application effect of digital micro-interactive teaching system in medical experiment,the digital micro-interactive teaching system is introduced into the experimental teaching of medical experiment,and the teaching method of computer experiment is used to promote the modernization of medical experiment teaching.The application of the digital micro-interactive teaching system for 12 years confirms that the system contributes to innovative,interactive and personalized medical experiment teaching.The management of digital multimedia microscope laboratory should be strengthened to build an open experimental sharing platform to cultivate innovative talents of outstanding excellence in medical science.the application of digital microscope mutual tutoring system in medical experiment can improve the teaching quality of medical experiment teaching.