Abstract: Based on the research achievements of organic light-emitting device(OLED)in recent years,an integrated experiment of preparation and characterization of OLED was designed.The experiment started from the device structure design and preparation process,covering the substrate cleaning,vacuum acquisition,masking skill,film thickness in-situ controlling,electrical-optical conversion efficiency calculation and analysis.The presented experiments effectively help the undergraduates to learn the frontier of material physics,semiconductor devices,optoelectronics and other fields has been fully expanded,which fully embodies the characteristics of multi-disciplines and the demonstration role of science and education collaboration.It is proven that the comprehensive experiment has achieved favorite results in practice teaching.