Abstract: In order to train qualified students to meet the needs of the purpose of experimental teaching,this paper tries to explore the teaching reform of microbiology technology experiment course.Improved the hardware conditions,increased the number of basic equipment,standardized management and resource sharing.The curriculum structure and form are optimized,the contents of experiment are integrated,and the scope of students’ autonomous experiment is expanded.A new assessment and evaluation system has been established,which combines experimental design,operation with basic theory examination,and has achieved good results initially.The initiative of students is improved,the initiative of research is improved,and the effect of experimental teaching is improved.The reform of experimental teaching has a positive effect on the training of students' abilities and the improvement of their comprehensive quality.