Abstract: Based on the structure characteristic of nano-materials and knowledge background of fluorescence and UV-vis,this manuscript synthesized Se/N-doped carbon dots and discussed the fluorescence property as a comprehensive research experiment,which relied on the instrument of the comprehensive training and teaching platform.Students can understand the relationship between the structures and the optical properties of carbon nanomaterials and the influence of the synthetic process on the properties of materials.It not only consolidates the basic knowledge of instruments and improves the operation skill of students,but also cultivates the comprehensive quality,innovative consciousness and ability of students.