Abstract: Taking the environmental microbiology experiment course as an example,this paper conducts exploratory research and practice on the mixed teaching of experimental courses in colleges and universities.The ideas of mixed teaching design of environmental microbiology experiment course are summarized,and the effects,problems and further reform directions of practical application are analyzed.Practice has proved that the mixed teaching mode organically integrates the advantages of online learning and face-to-face teaching mode,maximizes the teaching effect and provides an effective way to achieve the goal of higher education personnel training.