Abstract: This paper designed a comprehensive chemical experiment based on the catalysis of POMs and biomass energy. In this experiment, activated carbon was used as a carrier, and a series of catalysts with different concentration of H4SiW12O40 were prepared by impregnation method. They are characterized by infrared spectroscopy, and the catalyst was used to catalyze the transesterification of soybean oil with methanol to prepare biodiesel. The influence of reaction temperature, heteropoly acid concentration, catalyst dosage, reaction time and alcohol to oil ratio on biodiesel yield were investigated by univariate method. This experiment can be used as a comprehensive experiment and am open experiment for senior undergraduates, especially for undergraduates majoring in chemical engineering in agricultural and forestry universities.