Abstract: This paper briefly introduces the experimental scheme for the preparation and characterization of copper sulfate pentahydrate crystals in the university chemical experiment course. Compared with the traditional experiment, the crystal morphology was analyzed by recrystallization, component analysis and polarized light microscopy. As an example of comprehensive experimental project, this experiment integrates knowledge points of various disciplines. Aiming at some problems that still exist, a corresponding improvement plan is proposed. That is to say, the Diamond crystal simulation software is introduced, and the combination of macro and micro, the abstract theory is embodied, and the point of crystallography is deepened. Set up a cross-experiment project to consolidate students′ understanding of the basic principles of the microscope and operational specifications. Practice shows that the improved experiment enhances the students′ experimental skills and theoretical foundation, and lays a solid foundation for cultivating their research spirit and innovative ability.